Figure 1 (30K, color)
Figure 1 displays (a) reference video image (uncut specimen block
face) and (b) 2DG AR deformed due to slice
sectioning. Blue dots in both reference and AR images indicate the
location of control points used for initial vectors in
TPS registration and yellow dots in AR image indicate the final locations
of TPS warping solution achieved by cost
function minimization of MI metric. Note that some starting points
in AR image were markedly displaced.
Figure 2 (98K, b/w)
Figure 2 displays the result of MI registration of AR image with
the reference, in checker board pattern, using (a)
affine and (b) TPS warping transformation algorithms. While the affine
transformation (2a) results in remarkably good
matches for most internal structures of the brain, some features around
outer edges denote local deformations that
cannot be handled by the affine transformation, e.g. in the mid section
of the brain, top and bottom squares show
mismatches of outer edges and structures between hippocampus and cortex
as well as the lesion in frontal lobe.